کیت RT-PCR تک مرحله
RT PCR kit Component
10X Enzyme Solution 0.2 ml
2X qRT PCR Buffer 1.2 ml
Storage Condition
℃Store at 20
کیت RT-PCR تک مرحله
Description
AddProbe RT PCR Kit provides sensitive and easy to use components which contain all the reagents
for first strand cDNA synthesis and PCR reaction in one tube by using TaqMan® , and is designed for
high sensitivity and specificity on various real time instruments. Especially, thermostable MMLV RTase
(RNase H –), hot start Taq DNA Polymerase and RNase Inhibit or are included in 1 0 X Enzyme Solution
Quality Control
The performance of Add Probe RT PCR Kit is tested in an RT and PCR one tube reaction using human
total RNA with specific primers and TaqMan® probes . The sensitivity of the kit is verified by the detection of GAPDH and Actin transcript in 10 pg total RNA after 30 cycles. Storage and Stability
Add Probe RT PCR Kit is stable for 2 year s when stored in a constant temperature freezer at less than
℃20
[Note] 50x ROX dye ROX dye can be included in the reaction to normalize the fluorescent reporter signal, for instruments which are compatible with that option. 50x ROX is a 25
µM concentration. Use the following table to determine the amount of ROX to use with a particular instrument
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